* Ignite has long duration damage-over-time and prevents Io from healing his Tethered ally.

* Nature's Prophet treants can easily cleared by damage-over-time Ignite or Multicast Hand of Midas earning lots of gold.
* Fireblast and Unrefined Fireblast prevents or cancels out Nature Prophet from escaping with Teleportation.

* Spirit Breaker can chase Nature's Prophet from anywhere on the map with Charge of Darkness, making it hard for Nature's Prophet to split-push.
* Charge of Darkness and Nether Strike gives vision of Nature's Prophet when he tries to Sprout himself and teleport away.
Additional:
* Charge of Darkness allows Spirit Breaker to catch Nature's Prophet out when he tries to split push.
* Nature's Prophet can't hide in his Sprout against Spirit Breaker, as his Charge of Darkness provides vision on its target.
* Spirit Breaker can easily cancel Nature's Prophet's Teleportation.

* Charge of Darkness can be used to escape or counter-initiate against a Relocate gank.
* Greater Bash and Nether Strike is devastating due to Io's low armor, and can even knock it away from tethered target.

* Nature's Prophet's low mobility and health pool allows Phantom Assassin to destroy him in the early game.
* Phantom Strike allows Phantom Assassin to jump in and out of Sprout, provided she has vision.

* Phantom Strike allows Phantom Assassin jump directly to Io.
* Coup de Grace makes short work of Io's low health and armor.

* Heroes with invisibility: (Riki, Clinkz, Nyx Assassin

Additional:
* Nature's Prophet is often by himself. Clinkz can use invisibility to hunt him down and kill him, although a Quelling Blade may be needed to cut through his Sprout. Orchid Malevolence may also be needed if 3-4 seconds are not enough time to kill him, as he can teleport away.
* Clinkz can match Nature's Prophet's ability to split push.

* Strafe and Searing Arrows allow Clinkz to quickly kill Io due to its low armor.

* Heroes that have AoE damage: Lina, Keeper of the Light, Timbersaw, Leshrac, Bristleback, Axe

* Lightning Storm will usually hit both Io and its tethered target since both will be close enough for the lightning to jump.
* Diabolic Edict and Pulse Nova hit all units near Leshrac, meaning he can do colossal damage to Io and its tethered target.
Additional:
* Lightning Storm will usually hit both Io and his Tethered target since both will be close enough for the lightning to jump. It can then be followed up by Split Earth.
* Diabolic Edict and Pulse Nova hit all units near Leshrac, meaning he can do colossal damage to Io and his Tethered target.

* Broodmother, Nature's Prophet, Phantom Lancer by their nature offer very good target for Splinter Blast and Winter's Curse.

* Cold Embrace can render the extra attack speed from Overcharge meaningless.
* Splinter Blast and Winter's Curse are very affective against Io, as it has to stay close to its tethered hero.

* Crypt Swarm helps clear treant pushes.
* Silence prevents Nature's Prophet from using Sprout or Teleportation.
* Exorcism kills all treants.
Additional:
* Exorcism can quickly kill any Treants or Necronomicon units. Silence also prevents him from using Teleportation to escape or Sprout to lock you down.

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer

* Glimpse directly negates Relocate.
Additional:
* Disruptor can always Glimpse back Io or his target after he Relocated nearby.
* Even better, thanks to the indicator on the ground, you can even preemptively drop Static Storm and Kinetic Field on the spot they will relocate to.
* Thunder Strike and Glimpse are also good tools to bring back an Io that Tethered away in the fog of war.

* Heroes with detection: Bounty Hunter, Disruptor, Razor, Slardar, Spectre, Spirit Breaker

* Heroes that have AoE damage: Lina, Keeper of the Light, Timbersaw, Leshrac, Bristleback, Axe

* Quill Spray is very effective against Io, as it has very low armor and has to stay close to the fight to not break its Tether.

* Moon Glaives and Lunar Blessing help Luna to tear off Nature's Prophet's treants.
* Luna can easily cancel Teleportation with her Lucent Beam.

* Lucent Beam can cancel Tether and Relocate.
* Moon Glaives and Eclipse make short work of Io and its tethered ally.

* Heroes with invisibility: (Riki, Clinkz, Nyx Assassin

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper

* Heroes with detection: Bounty Hunter, Disruptor, Razor, Slardar, Spectre, Spirit Breaker

* Fragile heroes: Bounty Hunter has decent nuking capabilities with Shiruken Toss and Jinada thus making fragile heroes easy picks for ganking. Few examples of such heroes are:Crystal Maiden, Pugna, Skywrath Mage, Io, Witch Doctor, etc.

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer

* Heroes that have AoE damage: Lina, Keeper of the Light, Timbersaw, Leshrac, Bristleback, Axe

Additional:
* Berserker's Call and Counter Helix will still hit targets inside of a defensive Sprout.
* Counter Helix clears treants in no time.
* Nature's Prophet's low damage is unlikely to hurt Axe until the very late game.
* Nature's Prophet's low mobility makes him very easy for Axe to chase down or initiate on, especially when affected by Battle Hunger.

* Broodmother can destroy Nature's Prophet's treants with Spawn Spiderlings and summon spiderlings in their place.
* Broodmother loves buying Orchid Malevolence, which can prevent Nature's Prophet from escaping with Sprout and Teleportation.
Additional:
* Sprout cannot protect Nature's Prophet if Broodmother puts down a web.
* Nature's Prophet's low mobility means he is defenseless against Incapacitating Bite.
* Spiderlings absorb much of the damage from Wrath of Nature.

* Thirst allows Bloodseeker to see where Io relocated to, should either Io or the tethered hero have low health.
* Rupture forces Io to stay in place, preventing it from using the Tether bonus movement speed to escape.

* Nature's Prophet can literally do nothing against Spectre, because she can chase him from anywhere with Haunt.
* Spectre, just like Naga Siren loves buying Radiance, which can kill Nature's Prophet's treants, and grant her farm simultaneously.
Additional:
* Spectre can get to him anywhere on the map with Haunt.
* Spectre can pass through his trees with Spectral Dagger.
* Desolate's damage will melt him quickly, as he's alone most of the time.

* Undying's Tombstone will cheerfully chew through treants, incidentally netting you some extra income during fights.
* The above interaction tends to ensure that skirmishes are heavily populated with units, thereby buffing Soul Rip

* Clockwerk's long range initiation and catch makes it incredibly difficult for Nature's Prophet to split push and show on the map alone.

* Nature's Prophet has no innate way to deal with Gravekeeper's Cloak or kill {U:Familiar}s effectively.
* Familiars can fly over the trees created by Sprout. They can also use Stone Form when the Prophet is trying to use Teleportation.
Additional:
* Nature's Prophet has no natural method of dealing with Gravekeeper's Cloak and Familiars.
* Familiar's low base attack time can chip away Nature Prophet's health.
* Familiar's Stone Form destroys trees created by Sprout.
* Familiars can scout woods and foggy areas where Nature's Prophet is trying to use Teleportation.

* Once caught in Bear's Entangle, Nature's Prophet cannot escape with Teleportation.

* Ice Blast prevents Io from healing the target.
Additional:
* Ice Blast prevents Io from healing an ally with Tether and prevents healing from Overcharge
* Io is a common Mekansm builder which is useless if he is affected by Ice Blast.

* Astral Spirit can give Elder Titan a lot of damage and movement speed if Nature's Prophet stands close to his treants.
* Not only Echo Stomp can also prevent Nature's Prophet to escape Teleportation, but also, Elder Titan can also use Astral Spirit to bypass Sprout and use its own Echo Stomp.
* Aghanim's Scepter upgraded Earth Splitter can clear Nature's Prophet's treants and disarm him simultaneously, which can shutdown his pushing.

* Sprout will not protect Nature's Prophet from Huskar if he has already locked on with Life Break.
* Even a farmed Nature's Prophet is usually not durable enough to fight Huskar alone.

* Mass Serpent Ward can kill lots of treants if played defensively.
* Mass Serpent Ward, Hex and Shackles can catch and kill a Nature's Prophet trying to use Teleportation.
Additional:
* Shadow Shaman's Mass Serpent Ward together with Ether Shock can easily kill lots of Treant if played defensively.
* Mass Serpent Ward, Hex and Shackles can disrupt Nature's Prophet's escape attempt with Teleportation. Though he can Sprout himself.
* Nature's Prophet also has no ways of canceling Shackles.

* Treants increase Echo Slam's damage.
* Earthshaker's AoE stuns can hit Nature's Prophet through Sprout, without having direct line of sight, in order to interrupt Teleportation.
Additional:
* Echo Slam can do a lot of damage if Nature's Prophet stands close to his treants.

* Tinker with Rearm and Boots of Travel can teleport and defend towers whenever Nature's Prophet tries to push.
* Laser prevents Nature's Prophet from attacking. Additionally, even with Monkey King Bar, Nature's Prophet cannot hit towers and buildings.
* March of the Machines can easily kill an entire wave of Treants, giving Tinker lots of gold.

* Just like Batrider, his Ball Lightning can be used clear Nature's Prophet's treants and easily destroys Sprout.
* Electric Vortex prevents Nature's Prophet's escaping from Teleportation.

* Firefly can be used to clear Nature's Prophet's treants and allowing to jump on Sprout.
* Flaming Lasso prevents Nature's Prophet escaping from Teleportation.

* Jakiro's Macropyre can help him to escape from Sprout and can disrupt Nature's Prophet's jungling.
* Jakiro can disrupt Nature's Prophet's escape attempt with Teleportation with Ice Path.
Additional:
* Jakiro's skill arsenal can ruin Nature's Prophet's gameplay: Dual Breath and Liquid Fire can clear treants from Nature's Call while simultaneously slowing Nature's Prophet, Ice Path can disrupt Nature's Prophet's escaping attempt with Teleportation and Macropyre can not only clear treants from Nature's Call, but also can free Jakiro from Sprout.

* Sand King has many tools to deal with Nature's Prophet's treants.
* Sand King also can interrupt Teleportation with Burrowstrike.
Additional:
* With Caustic Finale, Sand King can easily kill the treants from Nature's Call.
* Burrowstrike allows Sand King to prevent Nature's Prophet from using Teleportation inside of his Sprout.

* Naga Siren is a natural split-pusher. With a Radiance equipped, she will easily out-push Nature's Prophet, farming his treants in the process.
* Ensnare prevents Teleportation. Even if he hid inside Sprout, Song of the Siren lasts long enough to prevent him from escaping.
Additional:
* Naga Siren is a natural split-pusher. With Rip Tide, she will easily out-push Nature's Prophet, farming his treants in the process.
* Ensnare prevents Teleportation. Even if he hides inside Sprout, Song of the Siren lasts long enough to prevent him from escaping.

* Shadow Fiend can easily farm his treants and get easy Necromastery Stacks.
* If Shadow Fiend buys Boots of Travel and Shadow Blade, he can counteract Nature's Prophet's split-push.

* Sleight of Fist with Battle Fury deals a lot of damage to Nature's Prophet's team if they stand close to his Treants, as well as killing all of his Treants and getting lots of gold.
* Ember Spirit with Fire Remnant and Boots of Travel can teleport and defend tower whenever Nature's Prophet tries to push.
Additional:
* Ember Spirit can easily deal with Nature's Call by using his Flame Guard and with Battle Fury. He also has multiple ways of escaping Sprout. Additionally, Ember Spirit can break Sprout's trees with Activate Fire Remnant and interrupt Teleportation with Searing Chains.

* Grimstroke can catch Io and his ally easily with SoulBind as Io has a tendency to tether himself with an ally frequently.
* Io is highly vulnerable to disables. Hence, Grimstroke's Phantom's Embrace is a great ability to hinder Io's potential.
Additional:
* Soulbind can target both Io and its Tethered ally, allowing Grimstroke and his team to target both with single target spells. Should Io try to Relocate, Phantom's Embrace can stop its channel to prevent escape.

* Heroes with detection: Bounty Hunter, Disruptor, Razor, Slardar, Spectre, Spirit Breaker

* Most summons and illusions can barely hurt Tidehunter at all, after their attacks get reduced by Anchor Smash and blocked by Kraken Shell: Nature's Prophet, Enigma
* Also Ravage's large area of effect and damage can easily damage all those units at once, or just straight up clear them.

* Heroes with detection: Bounty Hunter, Disruptor, Razor, Slardar, Spectre, Spirit Breaker

* Heroes with invisibility: (Riki, Clinkz, Nyx Assassin

* Heroes with cleave damage: Sven, Magnus, Kunkka

* Anti-Mage's high mobility from Blink and tendency to build Battle Fury makes him strong against split-pushing heroes: Nature's Prophet, Tinker, and Arc Warden.

* Heroes with summons can find their summons eliminated easily by Lina, like Broodmother, Nature's Prophet, and Chen.

Additional:
* Lina can easily prevent Nature's Prophet Teleportation escape attempts with Light Strike Array.
* Lina's Dragon Slave and Light Strike Array can kill Nature's Prophet's treants and grant her lot of farm.
* Nature's Prophet doesn't like buying Black King Bar, which is only item that can help him against Lina.

* Squishy heroes (Io, Crystal Maiden, Lina...), as Earth Spirit can deal tons of damage with Magnetize.

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper

* Heroes that have AoE damage: Lina, Keeper of the Light, Timbersaw, Leshrac, Bristleback, Axe

Additional:
* Timbersaw can easily cut down trees from Sprout, making it very difficult for Nature's Prophet to teleport away or trap enemies.
* Timbersaw's abilities quickly clear out treants.
* Reactive Armor negates most of the damage of Nature's Prophet's treants, and Timbersaw can often allow himself to be attacked in order to heal himself.

* Heroes who lack burst damage will have a hard time against Mars: Phantom Lancer, Nature's Prophet, Lich.

* Heroes who have summons, which can be quickly cleared by his powerful area of effect skill: Nature's Prophet, Enigma, Lycan

* Squishy heroes, usually supports, like Io, Ancient Apparition, Keeper of the Light etc can die instantly from Tiny's magic burst.

* Heroes with low armor and little mobility, like Huskar, Io, etc. will also have an unfortunate fate.

* Heroes who produce many summons will have a hard time against Storm's Dispel Magic during teamfight: Broodmother, Enigma, Nature's Prophet.

* Mass summoning heroes: Broodmother, Nature's Prophet and Enigma are all able to mass-summon units, which Puck is able to clear with Illusory Orb and Waning Rift instantly, due to their summons having low health.

* Pounce also stops Nature's Prophet's Teleportation.

* Similarly, heroes with low cooldown teleports can be afraid to split push against X Marks the Spot, such as Tinker and Nature's Prophet.
* In general, highly mobile heroes like Earth Spirit or Storm Spirit are at a disadvantage against X Marks the Spot.
* Similarly, heroes with low cooldown teleports can be afraid to split push against X Marks the Spot, such as Tinker and Nature's Prophet.

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer

* Heroes that have AoE damage: Lina, Keeper of the Light, Timbersaw, Leshrac, Bristleback, Axe

* Living Armor can be used to save a hero from a Relocate gank.

* Acid Spray and Greevil's Greed will quickly destroy pushes with treants.

* Doom can eats Nature's Prophet's treants with Devour and gains lots of gold.
* Doom usually buys Radiance or using his Scorched Earth deals a lot of damage to Nature's Prophet if they stand close to his Treants, as well as killing all of his Treants and getting lots of gold.
* Doom can pick up several different abilities from neutral creeps using Devour, some of which can be annoying to Nature's Prophet:
* Mud Golem, Dark Troll Summoner, Harpy Stormcrafter, Satyr Mindstealer, Satyr Tormenter and etc. are good examples.
* Doom and a ministun from Infernal Blade's prevents Nature's Prophet from escaping with Sprout and Teleportation.