* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer

* Doppelganger can dispel Leech Seed. It can also destroy large amounts of trees, counteracting Nature's Guise and Eyes in the Forest.

* Thirst allows Bloodseeker to see where Io relocated to, should either Io or the tethered hero have low health.
* Rupture forces Io to stay in place, preventing it from using the Tether bonus movement speed to escape.

* Heroes with invisibility will be detected by Thirst when they have low enough health: Bounty Hunter, Templar Assassin, Riki, Clinkz, Weaver, Treant Protector, Nyx Assassin.

* Fragile heroes: Bounty Hunter has decent nuking capabilities with Shiruken Toss and Jinada thus making fragile heroes easy picks for ganking. Few examples of such heroes are:Crystal Maiden, Pugna, Skywrath Mage, Io, Witch Doctor, etc.

* Invisibility reliant heroes: Heroes with invisibility abilities or those that have invested into invisibility items are hindered by Bounty Hunter's Track. Few examples of such heroes are:Clinkz, Riki, Treant Protector, etc.

* Spiderlings melt away Living Armor almost instantly.
* The vision from Eyes In The Forest may prove dangerous for Broodmother, however.

* Ice Blast prevents Io from healing the target.
Additional:
* Ice Blast prevents Io from healing an ally with Tether and prevents healing from Overcharge
* Io is a common Mekansm builder which is useless if he is affected by Ice Blast.

* Living Armor can be destroyed quickly with Ion Shell.

* Lucent Beam can cancel Tether and Relocate.
* Moon Glaives and Eclipse make short work of Io and its tethered ally.

* Lightning Storm will usually hit both Io and its tethered target since both will be close enough for the lightning to jump.
* Diabolic Edict and Pulse Nova hit all units near Leshrac, meaning he can do colossal damage to Io and its tethered target.
Additional:
* Lightning Storm will usually hit both Io and his Tethered target since both will be close enough for the lightning to jump. It can then be followed up by Split Earth.
* Diabolic Edict and Pulse Nova hit all units near Leshrac, meaning he can do colossal damage to Io and his Tethered target.

* Ignite has long duration damage-over-time and prevents Io from healing his Tethered ally.

* With Dual Breath, Liquid Fire and Macropyre Jakiro can quickly remove all Living Armor from a target.
* Ice Path allows Jakiro to prevent Treant Protector from using Overgrowth while staying at a safe distance.
* Macropyre destroy trees, which where Treant protector might be hiding

* Corrosive Haze will reveal Treant Protector even when he is under the effects of Nature's Guise.

* Glimpse directly negates Relocate.
Additional:
* Disruptor can always Glimpse back Io or his target after he Relocated nearby.
* Even better, thanks to the indicator on the ground, you can even preemptively drop Static Storm and Kinetic Field on the spot they will relocate to.
* Thunder Strike and Glimpse are also good tools to bring back an Io that Tethered away in the fog of war.

* Cold Embrace can render the extra attack speed from Overcharge meaningless.
* Splinter Blast and Winter's Curse are very affective against Io, as it has to stay close to its tethered hero.

* Lich's Chain Frost forces Io to give up providing buff to his ally with Tether or suffer devastating damage with his tethered ally.
* Lich could easily use Sinister Gaze to disable Io from getting away with Tether or Relocate.

* Not only are all of Timbersaw's active abilities capable of cutting down trees, but Whirling Death and Timber Chain also require it to be effective, counteracting both Treant Protector's Nature's Guise and Eyes in the Forest abilities.
Additional:
* Timbersaw can use his plethora of tree cutting abilities to level entire sections of the forest, making hiding in the trees more difficult for Treant Protector and his team.
* Timbersaw can easily cut down Treant Protector's Eyes In The Forest once his team has detection. He may even do it unknowingly due to the sheer destructive and chaotic nature of his abilities.

* Grimstroke can catch Io and his ally easily with SoulBind as Io has a tendency to tether himself with an ally frequently.
* Io is highly vulnerable to disables. Hence, Grimstroke's Phantom's Embrace is a great ability to hinder Io's potential.
Additional:
* Soulbind can target both Io and its Tethered ally, allowing Grimstroke and his team to target both with single target spells. Should Io try to Relocate, Phantom's Embrace can stop its channel to prevent escape.

* All of Treant Protector's abilities are worth stealing, and Rubick is able to cast them with no animation time.

* Light Strike Array and Dragon Slave allows Lina to destroy trees, countering Nature's Guise and Eyes in the Forest.
* Lina has many tools to remove Living Armor's protection.

* Quill Spray is very effective against Io, as it has very low armor and has to stay close to the fight to not break its Tether.

* Heroes with the capability of removing or destroying trees greatly hinders the effectiveness of Treant Protector's abilities: Batrider, Nature's Prophet, Lina, Enigma, Mars

* Heroes that wish to disrupt fights with spell combos or big ultimates are vulnerable to the long stun of Fiend's Grip: Disruptor, Death Prophet, Enigma, Faceless Void, Invoker, Lich, Magnus, Mars, Medusa, Outworld Destroyer, Phoenix, Puck, Queen of Pain, Shadow Fiend, Treant Protector, Underlord, Winter Wyvern, Witch Doctor, Zeus.

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper

* Squishy heroes (Io, Crystal Maiden, Lina...), as Earth Spirit can deal tons of damage with Magnetize.

* Heroes that can easily bypass Io's allies to get to Io: Riki, Tusk, Clinkz, Sniper

* Heroes with the capability of removing or destroying trees greatly hinders the effectiveness of Treant Protector's abilities: Batrider, Nature's Prophet, Lina, Enigma, Mars

* Heroes that are hard to gank: Axe, Death Prophet, Phantom Lancer

* Melee heroes with low mobility can be kited and killed by Viper: Treant Protector, Kunkka, Alchemist, Troll Warlord, Wraith King, Tiny, Sven.

* Heroes with the capability of removing or destroying trees greatly hinders the effectiveness of Treant Protector's abilities: Batrider, Nature's Prophet, Lina, Enigma, Mars

Additional:
* Firefly will cut trees with ease, cleaning-up an area that's been enchanted with Eyes In The Forest, and potentially revealing a Treant under the effects of Nature's Guise.
* Batrider's DoT spells are also good versus Living Armor,

* Squishy heroes, usually supports, like Io, Ancient Apparition, Keeper of the Light etc can die instantly from Tiny's magic burst.

* Strafe and Searing Arrows allow Clinkz to quickly kill Io due to its low armor.

* Monkey King is one of the few other heroes who can easily traverse through trees, counteracting Treant Protector's ability to hide in trees.